In the case of prolonged non-pregnancy, a medical history, physical examination, ultrasonography, hormone measurements, x-ray imaging, and tests such as basal body temperature measurement and semen analysis are required. A condition in which a couple has not been able to conceive after at least 12 months of uncontraceptive sexual intercourse is called infertility. When a woman is not pregnant, in addition to the woman’s own relevant examinations, the man also needs to carry out the necessary screening. 1. Understand the medical history: whether women have regular menstruation, amenorrhea, pelvic inflammatory disease, vaginitis and other diseases; men have erectile or ejaculatory disorders, mumps, prostatitis and other medical history. 2. Physical examination: whether there are abnormalities in the development of external genitalia and uterus. 3. Ultrasonography: women should focus on whether the size, position, shape, structure and thickness of the uterus are normal, and whether there are any abnormalities in the ovaries and follicles; men should check whether there are any abnormalities in the prostate, seminal vesicle glands, testicles and epididymis. 4. Hormone measurement: including blood follicle stimulating hormone (FSH), luteinizing hormone (LH), prolactin (PRL), estradiol (E2), testosterone (T), progesterone (P) and thyrotropin (TSH). To clarify whether there are endocrine abnormalities that subsequently affect pregnancy. 5. X-ray imaging: used to check whether the fallopian tubes are patent and the degree of patency. 6. Other tests: such as basal body temperature measurement, semen analysis to clarify whether there is azoospermia, dead sperm, weak sperm and other problems. If you are not pregnant, please follow the doctor’s instructions to carry out the necessary tests. In addition, relax your mind, part of the infertility is no clear cause.