In order to determine the site of the lesion, a three-cup urine test can be performed when clinically encountering hematuria and leukocyturia (pus urine). The procedure is as follows: after washing the vulva and urethral orifice, the first 10-20 ml of urine is left in the first cup, the middle 30-40 ml in the second cup, and the final 5-10 ml in the third cup, and the relationship between hematuria or pus urine and the sequence of urination is analyzed to find out from which part of the urinary tract it originates. Anterior hematuria or pus urine suggests that the lesion is in the anterior urethra, and terminal hematuria or pus urine suggests that the lesion is in the bladder neck and triangle or posterior urethra, etc.; full hematuria or pus urine suggests that the lesion is in the upper urinary tract (ureter and kidney) or bladder. 1, the first cup of urine, after urination begins to appear hematuria or pus urine, two cups of clear suggests lesions in the anterior urethra such as urethritis. 2, the first cup of urine and the second cup of urine clear, the third cup of urine appears red cells and pus cells, the end of urination appears in the blood urine or pus urine, suggesting that the lesion in the bottom of the bladder after the urethra or prostate parts, such as prostatitis, vesiculitis, etc.. 3, all three cups are cloudy or there is hematuria, suggesting that the lesion is in the bladder or above the bladder, such as pyelonephritis, glomerulonephritis, etc. 4, hematuria such as three cups of urine is uniformly blood-colored, microscopic examination are a large number of red blood cells, mostly seen in renal tuberculosis, kidney stones, nephritis, etc.; only the front segment hematuria, seen in urethral injury, tumors, prostatitis and meatus, etc.; only the posterior segment of the third cup of hematuria, seen in acute cystitis, bladder stones or tumor prostate lesions. 5, pus urine such as three cups of urine are cloudy, microscopic throughout a large number of pus cells, mostly seen in ureteritis, pyelonephritis, renal abscess, renal pus, kidney tumor co-infection, genitourinary system adjacent organs or tissue abscess to the urinary tract penetration; pus urine only in the first cup is seen in acute, chronic anterior urethritis; only the final pus urine, seen in prostatitis, vesiculitis, posterior urethritis, etc.. 6, according to the hematuria out of the different clinical site will be divided into glomerular and non-glomerular hematuria.