Male partner: 1. 2 semen tests, at least one semen test at our center. If you have a previous testicular puncture report or biopsy report, bring a copy of the routine semen examination and testicular puncture report. 2. Sex hormone test. 3, Hepatitis B carriers, need to check hepatitis B two to half and hepatitis B DNA, negative people only need hepatitis B surface antigen can be. (Valid for one year) 4.Hepatitis C (if positive, need to check hepatitis C RNA), AIDS, syphilis. (valid for one year) 5. blood type. Female: 1. Tubal imaging report or hysteroscopy report. 2.B ultrasound. 3. Sex hormones, third day of menstruation. 4, anti-sperm antibodies, anti-endometrial antibodies 5, chromosomes. 6, Hepatitis B, Hepatitis C, HIV, Syphilis, same as male partner test. 7.Blood type, blood routine, liver and kidney function. 8.White belt routine, cervical smear, gonococcus and urine routine.