The most reliable method to diagnose cervical cancer is cervical pathological biopsy. For early cases, TCT (liquid-based cytology), HPV (human papillomavirus), and colposcopic examination and evaluation are usually taken, which is completed by a three-step procedure of cervical biopsy, in which the definitive diagnosis is based on histological diagnosis. The cervical biopsy is a single or multiple point biopsy of the abnormal area under colposcopic examination and evaluation. For those who have abnormal TCT or HPV but have a smooth colposcopic surface with a type III transformation zone and no abnormality seen, cervical canal scratching may be performed at the same time to assist in the diagnosis. For individual patients whose cervical biopsy cannot rule out invasive carcinoma, or suspected microinvasive carcinoma, conization is usually required.