Introduction: Intrauterine insemination refers to a technique of collecting the husband’s semen and injecting it into the woman’s uterine cavity by non-coital means, i.e. by a physician to assist conception. 1.The physician in charge determines the indications for artificial insemination and excludes contraindications. 2.The nursing staff will review the three certificates (ID card of both parties, marriage certificate, birth certificate or certificate of not having children). 3.Laboratory examination for both male and female in one cycle in advance, requiring complete items. 4.Male side examination items: 3 semen routine examination (report card within one year, abstinence 3-7 days), sperm staining, acrosome enzyme; full set of secretion; blood routine; urine routine; blood type; hepatitis eight; HIV; syphilis antibody. Note: The above laboratory tests (except semen and blood type) are required within six months. 5.Female examination items: basic endocrine hormones (2-4 days after menstruation); full set of secretion; blood routine; urine routine; liver and kidney function; blood sugar; hepatitis eight items; HIV; syphilis antibody; eugenics four items; infertility eight items; blood type; tubal imaging or hysteroscopy or laparoscopy; gynecological ultrasound. Note: The above laboratory tests (except for tubal examination and blood type) should be performed within one year. 6.Establishment of medical record of husband-sperm artificial insemination; signing of informed consent for artificial insemination, informed consent for follow-up visit, informed consent for reduction of multiple pregnancy. 7.Monitor the follicle development and estimate the date of IUI surgery according to the situation. 8.On the day of the operation, the couple will sign the “IUI Clinical Operation Record” at the same time, and the male partner will receive a sperm collection cup marked with the couple’s name, number and date to the sperm collection room for sperm collection. After taking semen, hand the semen cup to the laboratory personnel in the indoor transfer window (both couples are present at the same time) and confirm by fingerprint on the signed “Artificial Insemination Clinical Operation Record Sheet”. 9.During the process of semen processing by the laboratory personnel, the couple goes to pay the artificial insemination fee. 10.After the semen is processed, the female partner empties her bladder and enters the operation room. 11.Sign on the laboratory register before the operation. Before injecting the semen into the uterine cavity, the medical personnel check with the woman the names and numbers of both spouses on the test tube. After the operation, lie flat in the operating room for 30 minutes again. After leaving the operating room, ask the physician in charge about precautions and follow-up consultation.