Human growth hormone is secreted in bursts of pulse-like secretion, with five to nine peaks per day, most of which occur at night during sleep. It can be seen that the concentration of growth hormone in human blood is changing. In addition, there is no difference between the basal values of blood growth hormone in children with growth hormone deficiency and normal children, and the growth hormone level taken from normal children early in the morning is also low. Therefore, doctors cannot judge whether the child has growth hormone deficiency based on the results of this one measurement of growth hormone. So, what kind of tests can doctors use to determine whether a child with dwarfism has a growth hormone deficiency? In addition to the pulse-like secretion of human growth hormone, its secretion is also influenced by factors such as exercise, food intake and stressful stimuli. The pituitary gland can be stimulated to secrete growth hormone by painful stimuli, hunger and hypoglycemia, as well as by certain medications. Therefore, based on these characteristics of growth hormone, doctors have devised various methods to check growth hormone levels in the body, such as screening tests (exercise tests and sleep tests, etc.) and many drug stimulation tests. Some of the commonly used drug-induced tests for growth hormone in clinical practice are: insulin hypoglycemia test, arginine, levodopa and colistin. These tests need to be performed early in the morning on an empty stomach in a quiet state, and a blood specimen needs to be collected every half hour for a drug provocation test, and at least two different drug provocation tests need to be performed. During these tests, children may have some adverse reactions, such as dizziness, vomiting, and insulin hypoglycemia test, hypoglycemic symptoms such as sweating and trembling may occur. Therefore, for safety reasons, children with dwarfism need to be hospitalized for the tests. When the results of 2 growth hormone stimulation tests with different drugs are low (both below 10 ng/mL), combined with the other clinical symptoms and examination results of the dwarf child, the doctor can make a comprehensive judgment that the dwarf child is growth hormone deficient.