This test was performed to compare intestinal fatty acid binding protein with various biological indicators to determine its feasibility for early diagnosis of intestinal ischemia. Methods Twenty adult healthy SD rats were divided into experimental group I (=10) and control group II (=10), 10 rats each weighing 250-300 g. Each SD rat was anesthetized by intramuscular injection of i0% chloral hydrate 5 mg/kg. In the control group, the abdominal cavity was opened only under aseptic conditions; in the experimental group, the abdominal cavity was opened along the midline, the root of the superior mesenteric artery was freed, the tongue was clamped with a noninvasive arterial clip for 60 min, and the noninvasive arterial clip was released and reperfused for 60 min. Venous blood was collected before surgery, 15, 30 and 60 min after surgery of the tongue, and 30 and 60 min after removal of the noninvasive arterial clip, respectively. The intestinal tissue was also scored by light microscopy. The results of intestinal fatty acid binding protein intestinal ischemia 15 min can reach ten times of the control group, ischemia 30 min up to the peak, reperfusion injury 30 min Yue decline, but still hundreds of times the serum concentration of the control group, there is a significant difference between the two groups (P < 0.001). Serum CK: The peak was reached at 30 min of ischemia in the experimental group, which was about 3-4 times that of the control group. Serum GOT: the experimental group gradually increased with time, peaked at 60 rain of ischemia and gradually decreased at reperfusion. There was no significant difference between each period and the control group. Serum LDH: there was a difference between the two groups within 60 min of ischemia (P<0.05): there was no significant difference in the degree of intestinal mucosal injury between the experimental group and the control group within 60 min of ischemia, and a difference appeared between the two groups in the occurrence of reperfusion injury Yue (P<0.05). Conclusion Intestinal fatty acid binding protein can be used as a more ideal biological indicator of early intestinal ischemia.