1, abstinence time 3-7 days, abstinence time is too long will affect the vitality, too short will affect the density. 2, to ensure the integrity of the semen sent for testing, semen is a mixture, especially the anterior segment semen quality is high, if the loss of this part of the semen will have a greater impact on the results of the semen routine analysis; attention to aseptic operation when taking sperm, hand holding the outer layer of the sperm cup, can not take the inner wall of the sperm cup; line of artificial assisted reproductive technology to take a shower and change underwear a day before the sperm; specimens should be sent to the laboratory within 1 hour after collection; the temperature should be maintained at 20~40℃. 3. It is best that the initial examiner should have two semen analyses, and the interval between two semen collections should be greater than 7 days, but not more than 3 weeks. If there is a significant difference between the two results, the specimen should be taken again for a third analysis.
If the semen is abnormal, further additional tests will be required. If there is no abnormality, the female partner needs to do the examination in detail. 4. Indicators of semen routine: density is the first, and now our center adopts the fifth edition of the standard: greater than or equal to 15 million/ml. Next is viability, viability refers to a+b level sperm, natural conception and artificial fertilization depends on viable sperm. Viability refers to a+b+c grade sperm, while C grade sperm are alive but cannot move forward, natural conception and artificial insemination are not possible, and can only be useful when doing IVF. pH value: normal value between 7.0-7.8. Liquefaction time: usually within 60 minutes, more than 60 minutes is diagnosed as abnormal liquefaction. Abnormality rate: Regarding morphology: the 4th edition of the WHO manual normal morphology is sufficient as long as it is more than 15%; in the 5th edition of the WHO manual, normal morphology is sufficient as long as it is more than 4%, however, since there is no reference data for Asians in the 5th edition guidelines, many fertility centers still use the 4th edition criteria. The development of this criterion reflects three problems, one is that there are definitely differences between different ethnic groups for sperm quality; second, the sperm quality of men shows a decreasing trend. 5, the treatment course of oligozoospermia: the cycle of spermatogenesis is 70 to 74 days, specifically, it takes about 64 days for sperm to be released to the varicose sperm duct, followed by 2 to 12 days of maturation in the epididymis, so a course of drug treatment is about more than 2 months, during which time insist on continuous medication, according to the course of treatment, and uninterrupted medication, in order to have an effect.