Usually, the most common test for infertility is semen routine, in fact, there are sperm morphology test, acrosome reaction, chromosomes and so on, but in order to avoid excessive examination, save the economy of the examination, time cost and physical damage and so on, will not be all done at the beginning. Guangzhou Liwan District Hospital of Traditional Chinese Medicine Men’s Department sucks 9 1, semen examination should be how to step down step by step? When is it necessary to do the examination of sperm morphology, acrosome reaction, etc.? The first step: generally first check the semen routine, this can provide an overall semen status, help doctors find further direction to find the cause of the disease; The second step: according to the characteristics of the disease to check the semen in-depth aspects: such as indicators of infection, trace elements, antibodies, DNA fragmentation, acrosomal analysis, etc.; The third step: further, you may want to consider the gene, such as chromosomes and Y chromosome micro-deletions, and so on. This is like enemy reconnaissance or map zoom, need to step by step in-depth, layer by layer, according to the significance of each of the following tests, combined with the principle of individualized analysis to develop. So especially remind patients, need to buy a plastic hard leather file bag to protect your each and every test results (regular hospitals have standardized processes, combined with the unique experience of doctors), to see the infertility needs enough time, the information must be left before and after the easy to analyze the doctor, each test is not easy to say are “tears” ah; in addition, the test is not easy to say is “tears” ah; the test is not easy to say is “tears” ah. “ah; in addition, the sperm test need to abstain from sex for 3-7 days, you can go directly to the general simple clinic to open the check. What are the semen tests? What is the purpose of each test? (1) Routine semen: semen color, volume, sperm concentration, semen liquefaction time, semen acidity and alkalinity (PH value), sperm motility (percentage of forward-moving sperm or a+b class sperm), survival rate and morphology, round cell concentration. Purpose: To understand the general condition of the semen, and to make a preliminary judgment on whether there is a “hostile situation”, or in which area it may be. (2) Seminal plasma biochemistry: mainly includes neutral alpha-glucosidase and fructose, carnitine, acid phosphatase, lactate dehydrogenase. Purpose: To help in the understanding of the function of the epididymis, prostate, and seminal vesicle glands. Neutral alpha-glucosidase and fructose reflect the secretion function of the epididymis and seminal vesicles and their patency respectively, and are suitable for patients with azoospermia or severe oligozoospermia who are suspected of having vasovaginal obstruction; seminal plasma acid phosphatase is a marker of prostate function, and the test is of great significance to patients who have poor semen liquefaction; seminal plasma lactate dehydrogenase is a key enzyme for sperm motility, and the enzyme test can be used to assist in determining the quality of sperm and predicting sperm fertilization ability. This enzyme test can help determine the quality of sperm and predict the fertilization ability of sperm. (3) Sperm acrosome analysis: It mainly includes sperm acrosome integrity rate and sperm acrosome enzyme activity analysis. Purpose: To a certain extent, it reflects the ability of sperm to fertilize the egg cell. It is suitable for patients with unexplained infertility, infertility patients who are ready to do artificial insemination or in vitro fertilization. (4) Infectious indicators of semen: seminal plasma elastase test and semen leukocyte staining; related microbiological examination Purpose: elastase is a kind of protease secreted by neutrophils of lobulated nucleus in the process of inflammation, and it is a marker of inflammation in male reproductive tract, and the combination of this test and semen leukocyte staining can determine the existence of inflammation in the reproductive tract; the concentration of round cells in the semen routine is also considered as one of the indicators of semen infection; to know whether there is any urinary tract infection; to know whether there is any urinary infection. The concentration of round cells in routine semen is also considered as one of the indicators of semen infection; to know whether there are infections of the urinary and reproductive systems and their related pathogenic microorganisms (bacteria, viruses, spirochetes, mycoplasma, chlamydia, etc., commonly gonococcus, papillomavirus, adenoplasma, and Chlamydia trachomatis, etc.). (5) Anti-Sperm Antibody (AsAb) Test: The main test is the mixed antiglobulin test of semen (MAR test). Purpose: To detect patients with poor sperm viability, high sperm agglutination or unexplained infertility. (6) Sperm Nucleus DNA Fragmentation Analysis: Analyzes the percentage of spermatozoa with DNA fragments in the nucleus of the spermatozoa. Purpose: Suitable for infertile patients whose female partner has unexplained miscarriage and embryonic arrest, and infertile patients who are ready to undergo IVF treatment because a high rate of DNA fragmentation in the sperm nucleus indicates that the proportion of sperm with DNA fragmentation in the nucleus of the spermatozoa is too high, which may affect sperm-egg binding, embryo quality, and so on. (7) Seminal plasma trace element analysis: most commonly zinc test, phosphorus, etc. Purpose: For those with low sperm motility and high malformation rate. Zinc, as a cofactor of more than one hundred enzymes in the body, is an essential and important element related to human growth and development, and is closely related to the metabolic activities of the reproductive system. Seminal plasma zinc testing has a certain guiding significance in the treatment of male infertility. (8) Chromosomes and Y chromosome microdeletions: generally in two cases, it is also necessary to check the chromosomes: the male side of the multiple routine semen examination. Purpose: To find out whether there are congenital factors in male infertility patients, applicable to sperm density less than 10 million/mL; those with a history of more than two (including two) spontaneous abortions, especially early abortions (within 3 months of pregnancy). 3. If all the tests are normal, is it basically confirmed that the male partner has no problem? If all the above semen tests of the male partner are normal, and the sex life is normal, the time of sex is reasonable (reasonable coitus: start the day before ovulation, have sex every other day, 3 times in a row, usually can cover the ovulation period; usually regular sex, 1-2 times a week, do not because of the fertility of the “chaotic sex”). Chromosome, chromosome and Y chromosome microdeletion and thalassemia gene mutation and other genetic tests are also normal, although it can not be confirmed that the male fertility is absolutely normal, but this is the focus of screening should be shifted to the spouse; such as both sides are normal, it may be necessary to further analysis. If they are not normal, further analysis and diagnosis are needed in the direction suggested by the abnormality.