At present, there are five main methods for the examination of Chlamydia trachomatis, as follows: first, serological examination: mainly through the detection of anti-chlamydia trachomatis infection virus antibodies in the blood to diagnose, but this test is relatively one-sided, can not be diagnosed by a positive. Second, tissue culture examination: identification by immunofluorescence method can be confirmed. Third, antigen detection methods: such as the detection of Chlamydia trachomatis DNA at the break with PCR, sensitivity and specificity are relatively high, but more expensive, and prone to false positives. Fourth, cytological examination: cells are used to make smears with fluorescent markers for monoclonal antibodies. Fifth, cytological diagnostic test: cells from the lesion need to be taken for smear and then stained for examination.