Infertility is a very complicated disease involving both husband and wife, and because of the complexity of its causes, we often encounter unsatisfactory results in treatment, the root cause of which is the failure to accurately determine the cause of infertility and to prescribe the right medicine. Therefore, infertility examination and diagnosis are very important in the process of infertility treatment. Causes of female infertility: 1. Ovulation dysfunction, which is manifested by the absence of ovulation in the menstrual cycle, or although there is ovulation, the luteal function is not sound after ovulation. 2. congenital abnormalities in the development of reproductive organs or acquired reproductive organ lesions that hinder the smoothness and function of the reproductive channels from the vulva to the fallopian tubes, preventing the sperm from meeting the egg and leading to infertility. 3. Immunological factors: The presence of anti-sperm antibodies in the female reproductive tract or serum causes sperm to agglutinate with each other, lose vitality or die, resulting in infertility or sterility. In addition, some infertile women have antibodies to their own eggs in their serum, which can prevent sperm from penetrating the egg for fertilization and can cause infertility. Infertility caused by sexual disorders, lack of sexual knowledge, systemic diseases and unknown causes account for about 1/3 of the causes of infertility. Female infertility examination is summarized as the thirteen screening techniques for women, namely vaginal examination, cervical examination, uterine examination, fallopian tube examination, ovarian examination, pelvic examination, endocrine examination, sex hormone examination, immune factor examination, systemic factor examination, mental and psychological factor examination, congenital factor examination and ovulation function examination. The details of female infertility examination are as follows: (1) Systemic examination In addition to the general examination, the reproductive system examination should also be done. In addition to general visual examination and palpation, vaginal speculum examination and internal examination (double or triple examination) should be performed to understand the general conditions of the vagina, uterus, cervix, fallopian tubes, ovaries and pelvis, such as whether the size and position of the uterus are normal, whether there are masses and pressure pain in the fallopian tubes, uterus and ovaries, whether there is painful lifting of the uterus, and the mobility of the adnexa. (2) Presumption of ovulation and prediction of ovulation period: can be determined by basal body temperature measurement as well as cervical mucus examination or hormone measurement. (3) Endometrial examination: If necessary, the functional status of the endometrium can be understood by biopsy, and this examination is a reliable method to understand the presence or absence of ovulation or the functional status of the corpus luteum, as well as to understand the size of the uterine cavity and to exclude uterine cavity pathologies such as tuberculosis. (4) Endocrine function measurement: for example, serum estrogen and progesterone levels are measured at different times of the menstrual cycle to understand ovarian function; basal metabolic rate is measured to understand thyroid function; adrenal function tests and serum lactogen measurement are performed. (5) Tubal patency examination: including tubal ventilation or fluid examination and uterine, mainly to understand whether the fallopian tubes are patent or not, as well as whether the uterine tubes are developing normally and whether there are deformities. For those with poorly patent fallopian tubes (e.g. mild adhesions), it is also useful for treatment. (6) Immunological examination: To understand the presence of anti-sperm antibodies, in addition to anti-sperm antibody measurement, it can also be indirectly understood through post-coital test, in vitro sperm penetration test, etc.