1, irreversible azoospermia (mainly refers to the primary spermatogenic failure caused by various diseases. Commonly, such as Creutzfeldt-Jakob disease; azoospermia; testicular descent dysplasia; testicular tumor; only support cell syndrome; germ cell dysplasia; various causes of spermatogenic blockage, etc.) and patients with severe oligospermia, weakness and teratogenesis (refer to ICSI indications). 2, vas deferens recanalization failure (refers to various etiological causes of vas deferens obstruction, after surgery repeated semen analysis by centrifugation after no sperm detection). 3, ejaculation disorder (ejaculation disorder caused by various causes, such as various organic lesions such as radiotherapy or post-surgery-induced ejaculation). Among the above indications, except for irreversible azoospermia, other than intracytoplasmic single sperm injection (ICSI) can be used to obtain offspring with their own blood relatives. If the patient insists on waiving the right to assist conception through ICSI, an informed consent form must be signed before AID can be used. 4. The male partner and/or family has a serious hereditary disease that is not suitable for fertility. 5. Surviving newborns cannot be obtained from mothers and children with blood type incompatibility. In the initial stage of AID operation, our center only treats AID in patients with irreversible non-obstructive azoospermia (e.g. azoospermia caused by genetic diseases, epididymis and testes without sperm retrieval, significantly shrunken testes, patients with hypergonadotrophic azoospermia, etc.)