In this study, both clinical and subclinical types were used in a controlled study, thus further reflecting the progressive process of the effect of VC on testicular spermatogenesis. Although there was no significant change in peripheral blood sex hormone levels in the subclinical type of VC, the sperm count and viability had already started to decrease due to its damage to testicular spermatogenesis, while the number of deformed sperm increased, leading to more infertility. This also indicates that as the degree of varicocele increases, the more damage it does to the testes. Currently, the best treatment for VC infertility patients is surgery, and although semen quality improves after surgery, the conception rate is still not high. So is it possible to improve the conception rate after surgery by early detection of subclinical VC? Subclinical VC has blood reflux in the spermatic cord, but the varicose spermatic cord cannot be reached during physical examination, so it is easy to miss the diagnosis, so the diagnosis of subclinical VC is especially important. Strengthening the early diagnosis and early surgical treatment of subclinical VC has guiding significance for male infertility.