Persistent hyperinsulinemic hypoglycemia of infancy (PHHI) is the most common cause of persistent hypoglycemia in the neonatal period, with an incidence of 1 in 50,000-1.4 in 50,000. It is characterized by hypoglycemia due to abnormal insulin overproduction, and if the pathological process is not terminated in a timely and effective manner, it will lead to serious neurological sequelae. The treatment of this disease is mainly pharmacological and surgical. For children who fail pharmacological treatment, surgical removal of part of the pancreas is the only effective treatment. With the development of minimally invasive surgery, laparoscopic pancreatectomy has become a safe technique. However, there are few reports on laparoscopic pancreatectomy for PHHI, and this study investigates the feasibility and effectiveness of this technique by reporting the technique and results of laparoscopic partial pancreatectomy for PHHI.