Artificial insemination (AI) is an assisted reproductive technology (ART) that uses non-coital delivery of sperm into the female reproductive tract for the purpose of impregnating a woman. Artificial insemination (AI) is an assisted reproductive technology (ART) that uses non-coital delivery of sperm into the female reproductive tract for the purpose of conceiving a woman. According to the source of the sperm, AI can be divided into artificial insemination by husband sperm (AIH) and artificial insemination by donor sperm (AID) from a third party sperm. According to different insemination sites, such as vaginal, cervical canal, uterine cavity, fallopian tube and abdominal cavity, they are called intravaginal artificial insemination (IVI), intracervical canal artificial insemination (ICI), intrauterine insemination (IUI) and intrafallopian tube artificial insemination (IFI), respectively. 1. Indications Male factors: sexual dysfunction, mild weak sperm; ovulation disorders; unexplained infertility; endometriosis (mild to moderate); cervical infertility. 2. Contraindications: acute and chronic systemic diseases and inflammation of the reproductive tract; female partner’s obstruction of sperm-egg union due to tubal factors; female partner’s genetic diseases, serious physical diseases, mental and psychological disorders; history of birth of babies with congenital defects and confirmed to be due to female factors; female partner’s exposure to teratogenic amount of radiation, toxic substances, drugs and in the period of action; female partner’s bad habits such as alcoholism and drug addiction. 3. The conditions of semen that can be IUI for male partner with weak spermatozoa Sperm density ≥ 15 million/ml, forward motion sperm (a+b) ≥ 15%; conditions that can be IUI after semen treatment: ≥ 70% of class a sperm after upstream, ≥ 20 sperm/HPF, at least one side of the fallopian tube is open. Artificial insemination refers to the process of injecting male semen into the female reproductive tract by artificial means, so that the egg and sperm can be naturally fertilized and then pregnant. Artificial insemination is divided into: husband artificial insemination, donor artificial insemination and mixed artificial insemination, the following talk about husband artificial insemination before the consultation preparation: 1, to the hospital reproductive center after consultation, the doctor will determine whether the situation is suitable for artificial insemination, if eligible, to sign the informed consent; 2, the consultation need to bring three certificates: qualified marriage certificate, ID card, fertility certificate; 3, both sides of the pre-operative examination. Female partner: gynecological examination, ultrasound examination, five items of hepatitis B, HIV, HCV PRB/TPPA blood type, thyroid function, sex hormone examination, etc. Male partner: semen examination, five items of hepatitis B, HIV, HCV PRB/TPPA blood type, etc. 4. Ovulation monitoring for the female, on the day of ovulation, the male partner keeps the semen, and then injects it into the wife’s uterus after being optimized by the laboratory technicians to complete Artificial insemination.