Factors associated with the occurrence of non-anastomotic bile duct stenosis after liver transplantation and strategies to prevent and control it. Rapid completion of donor liver resection to reduce thermal ischemia time, preservation of the abdominal trunk and branches as much as possible to minimize arterial loss, adequate biliary lavage to drain as much residual bile from the liver as possible; heparinization of hepatic arteries before reconstruction by pressurized flushing with heparinized saline and clamping of donor hepatic arteries during the procedure, immediately after opening of the portal vein; prevention of acute and chronic rejection in the postoperative period, and attenuation of cytomegalovirus Infection rate. Taking transcystic balloon dilatation to treat non-anastomotic bile duct stenosis. In this group of 36 patients, 4 cases (11.1%) developed non-anastomotic bile duct stenosis, 2 cases were cured, 1 case was relieved, and 1 case died. The occurrence of non-anastomotic bile duct stenosis is mainly related to arterial loss, cold/heat ischemic injury, ischemia-reperfusion injury, cholestatic toxicity injury, immune injury, cytomegalovirus infection and other factors, and the treatment method is mainly transcystic bile duct balloon dilatation, and the serious cases often need a second liver transplantation.