The most common type of artificial insemination is intrauterine insemination (IUI), which is a method of monitoring the ovulation cycle and injecting washed and selected sperm into the uterine cavity during the woman’s ovulation to increase the conception rate. Artificial insemination includes AIH (Artificial Insemination by Husband) and AID (Artificial Insemination by Donor). It is one of the most convenient and effective methods to assist conception because it enriches high quality sperm and removes a large number of impurities and inflammatory cells from the seminal plasma through artificial insemination, while directly injecting into the uterine cavity to avoid sperm loss in the vagina and cervix, resulting in a higher fertilization rate. Feminine insemination is mainly suitable for women with at least one open fallopian tube and normal follicle development after natural cycle or ovulation promotion medication, and for men with normal semen or mild oligozoospermia, sexual dysfunction or other unexplained infertility. In contrast, artificial insemination by donor sperm is suitable for patients with azoospermia or family genetic disorders. Here we briefly introduce the basic process of IUI: For first-time patients, the doctor will first understand the basic conditions of both men and women, their past medical history and previous treatment, and improve the relevant examinations to clarify whether you meet the indications for IUI. Once all the test results are taken, you can find a doctor for a second visit. For couples who meet the indications for IUI, you can establish your own IUI file with the examination results, marriage certificate and ID cards of both parties. Once the IUI cycle begins, your doctor will schedule your visit based on your menstrual cycle and ovulation and begin monitoring follicle development via ultrasound. During this process, some patients will use ovulation-promoting medications depending on the follicle development. When the dominant follicle is mature, between 48 hours before ovulation and 12 hours after ovulation, the husband performs semen collection by collecting semen in a sterile sperm collection cup and sending it to the laboratory for sperm washing. The processed sperm is sent through a hose into the woman’s uterine cavity. After confirming by ultrasound that the eggs have been expelled, luteal support therapy such as oral progesterone or Daphne is administered. 14 days later a blood test is performed to determine if pregnancy has occurred. IUI is usually recommended for 3-4 cycles and if no pregnancy occurs, in vitro fertilization-embryo transfer treatment is recommended. The following tests are required for IUI: Female partner: blood test, urine test, blood group, biochemistry, coagulation test, hepatitis B, hepatitis C, HIV, syphilis, prenatal viral test, triple A, reproductive hormone test, blood sedimentation, vaginal discharge, bacterial vaginosis, chlamydia, TCT, gynecological ultrasound. The female partner must have a tubal imaging and bring the imaging film to the doctor in charge. For male partner: routine blood, routine urine, liver function, blood type, hepatitis B, hepatitis C, HIV, syphilis, semen routine (3 times if abnormal) [check male ultrasound if necessary, etc.].