Artificial insemination is a non-coital method of transporting sperm into the female reproductive tract to conceive a woman, also known as IVF technique. Artificial insemination is mainly used for infertility caused by male aspects, such as severe hypospadias, retrograde ejaculation, erectile dysfunction, azoospermia, oligospermia, hypospermia, and semen indigestion. It can also be used for infertility caused by the female side, such as vaginal spasm, small cervix, abnormal cervical mucus, poor post-coital test, etc. During artificial insemination, sperm can be placed in different insemination sites, such as vagina, cervical canal, uterine cavity and fallopian tube, which are called intravaginal insemination, intracervical canal insemination, intrauterine cavity insemination and intrafallopian tube insemination, respectively. It is important to note that the semen removed during the operation needs to be tested and if the results show normal semen density and motility, the semen can be injected into the vagina, intracervical canal, uterine cavity or fallopian tube etc. with a syringe or catheter after the semen has been liquefied. Secondly, after IUI, the female partner should rest in bed for 2-4 hours with legs tightly closed and lower limbs slightly elevated to avoid semen flow. In addition, a comprehensive physical examination of the sperm donor is required before artificial insemination to exclude hereditary diseases. Secondly, if the woman suffers from systemic or infectious diseases, such as severe reproductive organ dysplasia and cervical diseases, artificial insemination cannot be performed.