1, the indications of artificial assisted reproductive technology 1, artificial insemination of husband sperm its indications are: (1) male infertility due to oligosperm, weak sperm, abnormal semen liquefaction, sexual dysfunction, genital malformation; (2) cervical factors; (3) reproductive tract abnormalities and psychological factors leading to infertility such as sexual intercourse; (4) immune infertility; (5) unknown causes of infertility. 2. In vitro fertilization-embryo transfer (IVF) is indicated for: (1) gamete transport disorders caused by various factors in the female partner; (2) ovulation disorders; (3) oligo- and hypospermia in the male partner; (4) endometriosis; (5) unexplained infertility; (6) immune infertility. The indications for intracytoplasmic single sperm microinjection are: (1) severe oligospermia, weak spermia and teratospermia; (2) irreversible obstructive azoospermia; (3) spermatogenic dysfunction (excluding genetic defects); (4) immune infertility; (5) in vitro fertilization failure; (6) sperm acrosome abnormalities; (4) embryo vitrification and thawing Embryo freezing is the process of freezing and preserving the remaining embryos from ovulation promotion cycles. In this way, when pregnancy is not obtained in the ovulation promotion cycle, ovulation can be stopped in the subsequent treatment cycles and pregnancy can be obtained only by transferring thawed embryos. By freezing and thawing the embryos, we can save money, maximize the use of embryos, and increase the cumulative pregnancy rate of a single ovulation treatment. 5. Assisted hatching Patients with advanced age, embryos that have been frozen or poor embryo quality may make embryo hatching difficult, which is one of the causes of embryo implantation failure. Therefore, giving assisted hatching to embryos that have difficulty hatching on their own can help improve the pregnancy rate. 6. Active immunotherapy for recurrent miscarriage There are many causes of recurrent miscarriage. If the cause of recurrent miscarriage is determined to be immune factors after examination, lymphocytes from the husband or a healthy third party can be injected to stimulate the mother to produce closed antibodies to protect the fetus from the maternal immune system and avoid miscarriage. The current treatment success rate of this therapy is over 80%. 7. Testicular biopsy and germ cell culture: Since the introduction of the intracytoplasmic single sperm microinjection technique in 1993, patients with no sperm in the semen but sperm in the testes and epididymis have gained hope for fertility. However, there is no good treatment for patients with little or no sperm in the testes and epididymis. Through years of research, we can make the number of sperm in the testicular tissue of patients with spermatogenic disorders increase significantly and make the original inactive sperm active through in vitro culture, making it possible for patients with azoospermia to use cultured sperm for in vitro fertilization to achieve fertility. 8.Male diseases We have rich experience in prostate diseases, sexual dysfunction and male infertility. In the past ten years or so, he has completed research on Chinese medicine preparations for fertility and stem cell treatment for male infertility, and has conducted in-depth research on low semen quality and azoospermia. The treatment of low spermatozoa can effectively improve the quality of semen. The specific items include (1) routine semen examination; (2) sperm morphological examination; (3) sperm cell membrane function examination; (4) sperm nuclear staining; (5) sperm penetration function test; (6) anti-sperm antibody test; (8) seminal plasma zinc level determination; (9) elastic scleroplasmase test; (10) acrosome enzyme test. The above tests provide a detailed understanding of the fertility function of sperm. Targeted treatment of the problems found in the tests can effectively improve semen quality. On weekdays, we perform routine semen examination and morphological examination, and perform tests (3)-(10) on Monday mornings. Since the (3)-(10) tests are complicated, it takes Wednesday morning to see the results. 2. Female infertility: specific items include: (1) ultrasound; (2) hysterosalpingography; (3) follicle monitoring; (4) diagnostic scraping.