I. Try to have the semen tested within 2-7 days of abstinence to ensure the accuracy of the test. (Semen discharge includes sexual intercourse, masturbation, seminal emission, and within 48 hours to 7 days after semen discharge). Preparation before sperm retrieval: ① Go to the bathroom to urinate first. ② Wash your hands. ③Clean the vulva. ④Check the name on the lid of the sperm extraction cup. Open the lid of the sperm extraction cup with the front side facing down. IV. Try to use the masturbation method for sperm retrieval. If there is difficulty in sperm retrieval, your wife can help or take the sperm outside the hospital, and if necessary, you can download relevant sexual videos or pictures in your cell phone. The sperm must be taken outside the hospital within 30 minutes to the laboratory, pay attention to insulation. V. Try to start ejaculation after the penis is erect and lasts for 5 minutes. 6. Ejaculate all the semen into the semen extraction cup. (Note: Tilt the cup slightly, place the urethral opening at the mouth of the cup and do not touch the inner wall of the cup). Seven, cover the sperm extraction cup. VIII. Pass the sperm extraction cup from the small window into the hands of the examiner and check the names again.