Vaginal cleanliness is the examination of vaginal secretions by laboratory personnel using a microscope to determine its cleanliness and the presence of specific bacteria and cells, etc., to determine the presence of inflammation in the vagina and also to further diagnose the cause of inflammation. Vaginal cleanliness is clinically divided into four degrees, which are determined by the number of vaginal bacilli, cocci, epithelial cells, leukocytes or pus cells, and can be explained as follows: degree I: vaginal bacilli predominate under the microscope, and a large number of epithelial cells are visible; degree II: some vaginal bacilli, epithelial cells can also be seen, and some pus cells and miscellaneous bacteria; degree III: only a small number of vaginal bacilli and epithelial cells are seen, but a large number of Ⅲ degree: only a few vaginal bacilli and epithelial cells, but a lot of pus cells and other miscellaneous bacteria; Ⅳ degree: no vaginal bacilli, almost all pus cells and a lot of miscellaneous bacteria. Cleanliness Ⅰ to Ⅱ degree is normal, Ⅲ to Ⅳ degree is abnormal, most of them may be caused by vaginitis or other inflammatory diseases, and pathogens such as pathogenic bacteria, fungi or trichomonas can often be found. In young girls or menopausal women, when ovarian function is insufficient and female hormone levels are reduced, vaginal epithelial proliferation is poor, glycogen is reduced, and vaginal bacilli are also reduced, making them susceptible to infection with miscellaneous bacteria, which can also lead to poor vaginal cleanliness and vaginitis. In conclusion, when the cleanliness of vaginal secretions is third degree and there are symptoms such as leucorrhea, odor and itching, it is necessary to treat with medicine in time.