1. Induction of superovulation Normal women only ovulate one egg per menstrual cycle in their natural state. The development and maturation of multiple follicles can be obtained through medication, which increases the success rate of surgical egg retrieval and in vitro fertilization. In order to evaluate the effect of ovarian stimulation and to decide the time of egg retrieval, usually 5 days after ovulation medication, vaginal ultrasound will be done to monitor the development of follicles, together with urine LH and blood estrogen level check, adjust the dosage and continue the medication until the day before egg retrieval. On the night of follicle maturation, inject 5000U of HCG and retrieve eggs 34-36 hours later. 3. Egg retrieval The eggs are aspirated through posterior fornix puncture under the guidance of vaginal ultrasound and transferred to culture fluid for 3 to 6 hours for fertilization. It is basically non-invasive. 4. Sperm optimization treatment The semen its optimized to screen for sperm with high motility and excellent fertilization ability. Semen should be retained at the same time on the day of ovulation. 5.In vitro fertilization and early embryo culture The oocytes are taken 2 to 6 hours after the egg and the optimally treated sperm are placed in the culture medium in a certain number of proportions to fertilize them in vitro. If the fertilized eggs are formed and develop normally, they will be transferred to the culture dish containing the growth solution and continue to be cultured for 1 to 3 days before embryo transfer. 6.Embryo transfer The well-developed embryos are put back into the uterine cavity through the transfer catheter so that the embryos can successfully implant in the uterine cavity and continue to grow and develop. After the transfer, lie down for 2 to 4 hours and avoid strenuous activities for 24 to 48 hours. 7. Post-transfer management Progesterone or HCG is given after transfer to promote the proliferation of luteal cells, to promote the synthesis and secretion of progesterone, and to support the luteal function. 2 weeks later, check for pregnancy, and if pregnant, use for the full 3 months.