It is common knowledge that chronic hepatitis patients visit hospitals, have blood drawn for laboratory tests and ultrasound done to understand the activity and severity of the disease, but it is not common knowledge that liver tissue specimens are taken for pathological examination through liver puncture to determine the activity of hepatitis, and the importance and necessity of liver biopsy is often overlooked. Liver biopsy is important because it is the most straightforward way to understand liver lesions with the least affected factors, and therefore it is the most accurate way to check for liver disease. In which cases is a liver biopsy necessary? For example, if you are infected with hepatitis B virus, the test result is major or minor triplet, some may be HBV-DNA positive, the liver function test is basically normal, you have no symptoms, but to determine whether the liver is really free of inflammation, it is impossible to rely on liver function, ultrasound and other tests, then only through liver biopsy can we clarify whether the liver is diseased. A comparative study between liver biopsy and liver function tests found that liver biopsy in patients with normal liver function for hepatitis B showed varying degrees of inflammatory changes in the liver in about 50% of patients, and in some cases, even early cirrhosis. In general, patients with non-inflammatory liver are not advocated for antiviral therapy because of the poor effect of antiviral therapy, and if liver biopsy is not performed, it is possible that some patients should be treated but are not treated. Liver puncture biopsy is the best option in jaundiced patients with long-term elevated serum transaminases and unclear etiology. When the diagnosis and treatment of chronic liver disease are difficult, liver aspiration biopsy is an important test to make an accurate diagnosis because it provides a direct understanding of the pathological changes in liver tissue.