Patient: Usually the machine is used to receive the drawn blood with one hand and the blood after cell extraction with the other hand. Please tell me more carefully? If the donor is a woman who has never given birth to a child, will it have any effect on her? Liu Jiajun, Department of Hematology, The Third Affiliated Hospital of Sun Yat-sen University: Your inquiry is about the collection of peripheral blood stem cells. The blood then enters the blood cell separator through a sealed sterile disposable collection device, and then the collected blood stem cells (mainly the cells marked as CD34 positive, which is the marker of stem cells) are collected into the blood storage bag, and the rest of the blood flows back to the donor’s body, and the total amount collected in the end is about 50-100ml. 100ml or so. Therefore, how can you collect these life-saving stem cells from a patient if you do not go through a machine collection and draw peripheral blood alone? There are usually no serious reactions or complications during the collection process, but occasionally a few people will feel numbness in their hands and feet, dry mouth or dizziness from the needle. We have performed donor peripheral blood stem cell transplantation for many years in our hospital’s hematology department and have not encountered any donors with any special discomfort. Therefore, there is no need for you to worry at all. Prospective investigations have proven that peripheral blood stem cell transplantation has no effect on the fertility of both male and female donors, but pregnant women are still prohibited from donating. The need for G-CSF injections prior to stem cell collection also poses some risk to the fetus. Therefore, women need to be tested for pregnancy before undergoing G-CSF injections.