The main purposes or uses of immunohistochemical pathological tests related to hepatocellular carcinoma in clinical practice are as follows: 1. To identify whether it is primary hepatocellular carcinoma, or intrahepatic cholangiocarcinoma, or a mixed type of hepatocellular carcinoma and intrahepatic cholangiocarcinoma, specific immunohistochemical markers are Arg-1, AFP, polyclonal CEA, CD10 and other indicators. 2. For determining whether it is early hepatocellular carcinoma, commonly used immunohistochemical markers are indicators such as GPC-3, HSP70 and glutamine synthetase.3. Immunohistochemical detection of PD-1 and PD-L1 in liver cancer tissues to determine whether immunotherapy with immune checkpoint inhibitors is appropriate for liver cancer.