The markers of hepatitis B virus are: surface antigen (HBsAg), surface antibody (HBsAb), e antigen (HBeAg), e antibody (HBeAb) and core antibody (HBcAb). This is commonly referred to as “two and a half pairs”. If the serum is positive for these markers, it means that you have been infected or are infected with hepatitis B. It does not mean that you have liver cancer. However, the “two-and-a-half” test is used in the diagnosis of liver cancer for the purpose of differential diagnosis of liver cancer. Since more than 90% of primary liver cancer patients are positive for hepatitis B virus markers, and epidemiological evidence has fully demonstrated the etiological relationship between hepatitis B virus and primary liver cancer, if substantial occupying lesions are found in the liver with positive hepatitis B virus markers, the diagnosis of primary liver cancer is supported; on the contrary, if hepatitis B markers are negative, unless evidence of hepatitis C is found, the diagnosis of primary liver cancer may be benign. evidence, then it may be a benign lesion or secondary hepatocellular carcinoma.