In what cases do male infertility patients need to be tested for sex hormones? 1.Abnormalities in medical history or physical examination. 2, Sex hormone test should be done when sperm density is <10×106. 3.If the initial screening results are abnormal testosterone should be rechecked and PRL and LH should be detected. 4.Because testosterone levels are higher in the morning, it is best to have blood drawn for sex hormone testing at 7~10 am.