Abstinence from sex for 3-7 days is usually required to check sperm quality, which helps the accuracy of the test results. Usually abstinence for less than 3 days or for more than 7 days may affect both sperm viability and sperm count, thus affecting the test results. Semen specimens need to be sent for examination immediately after collection, with a time frame of no more than one hour. Sperm viability has a great relationship with the external temperature. When the temperature is lower than 20°C, or higher than 40°C, it will affect sperm viability. In addition, the production of sperm varies greatly from day to day. If the test result is abnormal, it is necessary to test again in about 7-14 days and 2-3 consecutive tests to obtain the correct result. In addition, men should pay attention to quit smoking and drinking for 2-3 months before semen examination, get more rest, ensure sufficient sleep, and not stay up late. Eat regularly, strengthen nutrition, and do not use any medication 1 week before the test.