1, abstinence time is controlled between 2-7 days, too long will affect the vitality, too short will affect the density. But these effects are not absolute, most of them are. 2, the first examination, mainly first check the semen routine, sperm morphology, anti-sperm antibodies, if there are abnormalities, then further other examinations are required. If there is no abnormality, then the female partner needs to do the examination in detail. 3. Indicators of semen routine: density is the first, and now our center adopts the fourth edition of the standard: greater than or equal to 20 million/ml. Next is viability, viability refers to a+b level sperm, natural conception and artificial fertilization depends on viable sperm. Viability refers to a+b+c grade sperm, while C grade sperm are alive but cannot move forward, natural conception and artificial insemination are not possible, and can only be useful when doing IVF. pH value: normal value between 7.0-7.8. Liquefaction time: usually within 30 minutes, more than 30 minutes is diagnosed as abnormal liquefaction. Aberration rate: Although there is an aberration rate on the semen routine, it is not accurate enough and usually cannot be used as a judgment standard. What should be accurate is to check the morphology of sperm after staining. Now the normal sperm morphology according to the fifth edition of the standard, more strict, greater than or equal to 4 percent even if normal. 4, less weak sperm treatment course: this is determined by the process of sperm occurrence to maturity, generally speaking, sperm from occurrence to maturity takes about 70 days, that is to say, the treatment of a course of treatment for about more than 2 months, during which adhere to continuous medication, according to the course of treatment, uninterrupted medication, in order to have an effect.