1. Abstinence should be at least 48 hours, but not more than 7 days, before specimen collection. The optimal time is 3-5 days of abstinence. To reduce fluctuations in semen analysis results, the number of days of abstinence should be as constant as possible. Each semen analysis report should state: the patient’s name, the duration of abstinence, the date and time of specimen collection, whether the specimen collection is complete and the time interval between specimen collection and analysis. 2. The initial examiner should have two semen analyses, and the interval between two semen collections should be greater than 7 days, but not more than 3 weeks. If there is a significant difference in the results between the two times, the specimen should be taken again for a third analysis. 3. The collection of specimens should preferably be done individually in a sperm collection room near the laboratory. Otherwise, it should be sent to the laboratory within 1 hour after collection. 4. It is best to take semen by masturbation and collect semen in a wide-mouthed glass or plastic container that has no toxic effect on sperm. The temperature should be kept at 20~40℃ to avoid reducing sperm viability. If microbiological examination is to be done, the patient should first urinate and wash his hands and penis and collect in a sterile container. 5. If masturbation is difficult, special condoms can be used for semen collection. Because latex condoms used daily can affect the survival of sperm, they cannot be used for semen collection. The interruption of intercourse method also cannot be used for semen collection because the initial portion of the ejaculate, which often has the highest sperm density, may be lost. Moreover, the specimen will be contaminated by bacteria and microorganisms; at the same time, acidic vaginal secretions will also have a negative impact on sperm vitality. 6, Semen collection must be complete, incomplete semen should not be analyzed. 7. The temperature of the specimen should be kept above 20°C, but not more than 40°C, during transportation to the laboratory. 8, The container for semen collection must be marked with the name of the subject (and/or ID number) and the date and time of specimen collection. 9.If you have a recent history of fever or medication, it is best to avoid the examination.