Semen specimen retrieval precautions 1. Abstinence time: 2-7 days before semen examination (i.e. no more ejaculation within 2-7 days after the last semen discharge, the semen specimen within this period is suitable for semen examination). Abstinence for too short a period (less than 48 hours) or too long (more than 7 days) is not suitable for semen examination. If the abstinence time has exceeded 7 days, the semen should be discharged within 2-7 days and then come back for semen examination. 2. Specimen collection method: Semen should be collected by masturbation and semen should not be retained by condom 3. Complete collection: When collecting semen, try to completely ejaculate the semen in your body, otherwise, it will result in less semen retention than the actual semen volume. If the semen volume is not close to the real level of the detector, it will seriously affect the accuracy of the test results. 4, specimen collection: semen specimens should be collected intact in the designated container without any omission, otherwise the accuracy of the test results will be affected. 5. Specimen delivery: Semen should be sent for testing immediately after ejaculation. If semen is left outside the hospital, care should be taken not to contaminate the semen, the exact time of semen ejaculation should be recorded after semen removal, and the semen specimen should preferably be delivered within 30 minutes, with the longest time not exceeding 1 hour, and care should be taken to keep the semen specimen warm (20-40℃) during transportation when it is cold.