Gliomas are the most common primary malignant brain tumors in adults, and malignant gliomas in particular are common. Due to the diffuse infiltrative growth of malignant tumors, indistinct from surrounding normal brain tissue, and rapid progression, patient prognosis is poor. Although the extent of surgical resection has a significant impact on the survival of patients, the rate of complete resection is only about 1/3 based on the experience of tumor color and texture due to the diffuse infiltrative growth and unclear boundary with brain tissue, therefore, patients often recur rapidly after surgery, and it is often difficult to control the progression of the disease even with radiotherapy. For example, the average survival of glioblastoma patients is only 14.6 months, and the 5-year overall survival rate is only 9.8%. Our center adopts the latest international surgical microscope Pentero 900 equipped with YELLOW 560 special module to perform microsurgical resection of malignant glioma under the fluorescence guidance of sodium fluorescein, which can achieve intraoperative yellow fluorescence of the tumor but not the normal brain tissue (e.g. G figure), thus achieving precise surgical resection of malignant glioma under the surgical microscope while effectively protecting the normal brain tissue around the tumor. The microsurgical resection of malignant glioma can be achieved under the surgical microscope, while effectively protecting the normal brain tissue around the tumor.