Glioma: malignant tumors of the nervous system, ranking first in the incidence of cranio-cerebral tumors, spinal cord glioma is mostly seen in adolescents and has a relatively good prognosis. Glioma is a new organism formed by abnormal proliferation of glial cells. Because the pathological mechanism of its development is unknown, there is still no good treatment method. However, some scholars believe that glioma is a systemic disease, and once the lesion is found, it means that the glial cells in the whole nervous system have the possibility to be derived into tumor cells, so even though the combination of surgical resection, radiotherapy or immunotherapy is applied, the in situ recurrence of the tumor cannot be stopped. Some scholars even reported that the tumor may spread along the subarachnoid space, cerebrospinal fluid or invade organs outside the nervous system. With the advancement of stem cell research, it has been found that the abnormal proliferation and differentiation of tumors are associated with a small group of highly proliferative cells, tumor stem cells, and the original driving force for unlimited proliferation and differentiation of tumors comes from the activity of tumor stem cells. Such a cell population also exists in glioma tissues: glioma stem cells, which cannot be effectively killed by radiation, chemical agents, and immunological agents. Under the influence of external physical and chemical environment, these cells are temporarily latent, as reflected in in vitro experiments or some short-term in vivo experiments, where certain physical or chemical therapeutic measures are effective in the treatment of glioma. However, for the human body’s own physiological state and the function of various systems and organs, it is impossible to permanently withstand these physical or chemical stimuli or therapeutic interventions. After the physical and chemical treatments are stopped, the latent glioma stem cells, like “guerrillas”, will immediately take the stage again and proliferate and differentiate in a reactive manner until the tumor recurs. This is why we have a staged approach, which cannot eradicate glioma stem cells, but can effectively prolong the survival of patients. However, there are many physical and chemical methods for glioma, such as: X-ray, Y-rays, isotope particles (I125, 131, De99m, etc.), microwaves, hemorrhage, any gangue pepperǎ5-FU, ACNU, BCNU, CCNU, methotrexate, temozolomide, elemene, paclitaxel, cileol, bevacizumab, and other chemical drugs. Our proposed concept is to apply internationally recognized methods to extract this small group of dangerous molecules – glioma stem cells – and to individually screen in vitro and in nude mice for physical and chemical regimens that are specific and sensitive to each individual, and then to follow a fractionated containment strategy to achieve “permanent The aim is to “permanently” suppress the “resurgence” of glioma stem cells.