The prevalence of infertility in couples of normal reproductive age is about 15%. The incidence of female infertility due to male causes is as high as 50%. Sperm motility, especially the role and status of sperm with effective and linear motility, is more important in the reproductive process than sperm density and total sperm count, and sperm motility grading is more sensitive and effective than simple sperm motility measurement in male fertility evaluation. Sperm cell morphological examination Normal spermatozoa are like tadpoles, consisting of head, body and tail. Abnormal sperm morphological indicators of fertile spermatozoa are generally less than 30%. Staining examination of sperm morphology revealed that the rate of abnormal spermatozoa accounted for more than 59% of the number of people examined, and the rate of altered head abnormality was the highest among the various abnormal sperm morphologies, accounting for 42.4% of the sperm abnormality rate. A comprehensive analysis of the malformation rate and other semen indicators showed that an increase in the sperm malformation rate tends to lead to sperm viability disorders, and a significant proportion of individuals who may not have obvious abnormalities in other semen examination indicators have an increased sperm malformation rate, and it will be difficult to detect such patients simply by relying on semen routine. The semen of infertile men shows that the abnormal ratios of semen volume, sperm motility, sperm motility and malformation rate are very prominent, indicating that low sperm motility and excessive malformed sperm are important causes of male infertility. The routine semen examination only detects the physical and chemical properties of male semen and only makes a simple assessment of sperm motility, which cannot meet the needs of in-depth clinical diagnosis. It has practical guidance for the diagnosis and treatment of clinical infertility. The morphological staining of sperm is necessary clinically, and the physical and chemical indices of male semen and sperm morphological examination should be performed as a routine means of clinical infertility examination.