At present, there is no complete and uniform value for the determination of endocrine sex hormones in obstetrics and gynecology, and due to the source of various reagents, the method of determination, the calculation of data, and the use of different units, even for the same hormone specimen, the results obtained by various laboratories are not exactly the same. The following reference values of sex hormone tests are compiled with reference to various specialized books and magazines at home and abroad in the hope that they can provide useful reference for all colleagues. General knowledge of sex hormone test At least one month before the basic sex hormone test, sex hormone drugs (including progesterone, estrogen) can not be used, otherwise the results are unreliable (except for the need to review the sex hormone after treatment). Sex hormones can be checked at any time during menstruation, and the normal values are different at each time of the month. However, it is important to know the basal sex hormone level for the diagnosis and treatment of infertility. The first step is to choose the 2nd to 5th day of menstruation for the test, which is called the basal sex hormone level, and it is best to measure it on the 3rd day. To determine whether it is the 3rd day of menstruation, it is sufficient to check the 5 items of sex hormones, and it is possible not to check progesterone, which should be checked in the luteal phase (21 days after menstruation or 7 days after ovulation); however, it is not certain whether the vaginal bleeding is menstruation or not, and it should be checked in the 6 items, in order to prevent the misdiagnosis (according to the P data, it is possible to roughly determine the time period of menstruation cycle). Scarce menstruation and amenorrhea can also be done as a basal state if there is a negative urine pregnancy test, no ≥10 mm follicles in both ovaries on vaginal ultrasound, and an EM thickness of 5 mm. Basic sex hormone test should be seen in this way: the normal values of basic LH and FSH are 5-10IU/L, and the normal value of basic E2 is 25-50pg/ml (the results of these three items can not look at the reference value of the test sheet, according to this standard); PRL, T can be compared with the reference value of the hospital’s test sheet, and the normal value of P is shown in the back. Second, the clinical significance of sex hormone examination (a) FSH and LH: the basal value of 5 ~ 10IU / L normal menstrual cycle, follicular early (menstruation 2 ~ 3 days) blood FSH, LH are maintained at a low level, the rapid rise before ovulation, LH as high as the basal value of 3 ~ 8 times, up to 160IU / L or even higher, and FSH is only about 2 times the basal value of the value of very few 30IU / L, ovulation after the After ovulation, FSH and LH rapidly return to follicular phase levels. Monitoring FSH and LH levels in the early follicular phase can be used to initially determine the function of the gonadal axis, and FSH is more valuable than LH in determining ovarian potential. 1.Ovarian failure: basal FSH40IU/L, LH elevated or 40IU/L, for high gonadotropin (Gn) amenorrhea, i.e., ovarian failure; if it occurs before the age of 40 years, it is called premature ovarian failure (POF). 2.Basal FSH and LH are both 5IU/L for low Gn amenorrhea, suggesting hypothalamic or pituitary hypoplasia, and the difference between the two needs to be made with the help of gonadotropin-releasing hormone (GnRH) test. 3.Defective ovarian reserve (DOR): basal FSH/LH2～3.6 suggests DOR (FSH can be in the normal range), is the early manifestation of ovarian dysfunction, often suggesting that the patient’s response to super-ovulation (COH) is not good, should be adjusted in a timely manner COH program and the dosage of Gn in order to improve ovarian responsiveness, to obtain the desired pregnancy rate. Because elevated FSH/LH merely reflects DOR rather than decreased fertility, once the period of ovulation is obtained, the ideal pregnancy rate can still be obtained. 4.Basal FSH12IU/L, review next cycle, continuous 12IU/L suggests DOR. 5.Polycystic ovary syndrome (PCOS): basal LH/FSH2～3, can be used as the main indicator for diagnosis of PCOS (the basal LH level of 10IU/L is elevated, or LH maintains the normal level, while the basal FSH is relatively low level, which forms the elevated ratio of LH to FSH ). 6, check 2 times the basal FSH>20IU / L, can be considered to be premature ovarian failure occult stage, suggesting that 1 year after the possible amenorrhea. (ii) P: basal value is generally <1ng/ml Normally, blood P has been at a low level in the follicular phase, with an average of 0.6~1.9nmol/L, and is generally <10nmol/L (3.15ng/ml); P secretion begins to increase when the LH peak occurs before ovulation, and a large amount of P is produced by the ovarian corpus luteum after ovulation, with a rapid rise in blood P concentration; at the maturation of the corpus luteum (6-8 days after the LH peak), blood P concentration peaks; at the maturity of the corpus luteum (6-8 days after the LH peak), blood P concentration reaches the peak. When the corpus luteum matures (6~8 days after the LH peak), blood P concentration reaches a peak of 47.7~102.4nmol/L (15~32.2ng/ml) or higher, then decreases continuously and reaches the lowest level in the premenstrual period. The change of P content of peripheral blood in the whole corpus luteum is parabolic. 1.Determination of ovulation: middle luteal phase (the 21st day of menstruation for women with a 28-day menstrual cycle) P＞16nmol/L (5ng/ml) suggests ovulation, and 16nmol/L (5ng/ml) suggests no ovulation. 2.Diagnosis of luteal insufficiency (LPD): P32nmol/L (10ng/ml) in the middle of luteal phase, or 3 times of P measurement on the 5th, 7th and 9th days after ovulation, the total sum of 95.4nmol/L (30ng/ml) is LPD; or P47.7nmol/L (15ng/ml) before the 10th week of gestation is a criterion of diagnosis of LPD. 3.To determine the prognosis of in vitro fertilization-embryo transfer (IVF-ET): P level before ovulation can estimate the prognosis of IVF-ET. P ≥ 3.18 nmol/L (1.0 ng/ml) on the day of inotropic HCG injection should be considered elevated, with decreased implantation and clinical pregnancy rates, and P 4.77 nmol/L (1.5 ng/ml) suggesting premature luteinization. In IVF-ET long protocol ovulation, even if there is no elevation of LH concentration on the day of intramuscular HCG injection, if P (ng/ml) × 1000/E2 (pg/ml) > 1, premature luteinization of follicles is suggested, and the clinical pregnancy rate is significantly reduced in this group of patients. Premature luteinization is also a manifestation of DOR. 4, identify ectopic pregnancy: ectopic pregnancy blood P level is low, most patients blood P47.7nmol / L (15ng / ml). Only 1.5% of patients ≥79.5nmol/L (25ng/ml). In normal intrauterine pregnancy, P90% 79.5nmol/L and 10% 47.6nmol/L. Blood P level can be used as a reference in the differential diagnosis between intrauterine and ectopic pregnancy. (C) E2: basal value of 25 ~ 45pg / ml in the normal menstrual cycle, follicular early E2 is about 183.5pmol / L (50pg / ml), before ovulation reached the first peak, up to 917.5 ~ 1835pmol / L (250 ~ 500pg), after ovulation, a rapid decline, the luteal phase of the formation of a second peak of about 458.8pmol / L (124.80pg), and the second peak of about 458.8pmol / L (124.80pg), the luteal phase of the formation of a second peak, about 458.8pmol / L (124.80pg). 124.80pg), maintained for a period of time, luteal atrophy when it drops to the level of early follicular phase, i.e., on the 3rd day of menstruation it should be 91.75～183.5pmol/ml (25～50pg/ml). 1, Basal E2 >165.2 to 293.6 pmol/L (45 to 80 pg/ml), regardless of age and FSH, suggests decreased fertility. 2.When basal E2≥367pmol/L (100pg/ml), ovarian response is even worse, and there is no possibility of pregnancy even if FSH15IU/L. 3.Indicators for monitoring follicular maturation and ovarian hyperstimulation syndrome (OHSS) ①Promoting follicular ovulation: when promoting superovulation treatment, when the follicle is ≥18mm and the blood E2 reaches 1,100pmol/L (300pg/ml), discontinue the use of HMG, and inject HCG 10,000IU on the same day or 24-36 hours after the last injection of HMG. ②E2 ≥670pmol/L ( 1000pg/ml), OHSS generally does not occur. ③E29175pmol/L (2500pg/ml), a high-risk factor for the occurrence of OHSS, timely discontinue or reduce the dosage of HMG, and disable HCG to support the luteal function, which can avoid or reduce the occurrence of OHSS. When E214800pmol/L (4000pg/ml) is present, OHSS occurs in nearly 100% of the cases and can rapidly develop into severe OHSS. (4) PRL PRL is synthesized and secreted by the eosinophilic PRL cells of the pituitary gland. PRL secretion is unstable, and its secretion can be affected by mood, exercise, sexual intercourse, hunger and eating, and has small fluctuations with the menstrual cycle, with a It has a rhythmic nature related to sleep; PRL secretion increases in a short period of time after going to sleep, and is higher in the afternoon than in the morning. Therefore, according to this rhythmic secretion characteristics, blood should be drawn on an empty stomach at 9~10 a.m. If PRL is significantly elevated, it can be determined by a single examination; if PRL is mildly elevated, a second examination should be performed, and the diagnosis of hyperprolactinemia (HPRL) should not be easily diagnosed with the abuse of bromocriptine treatment. PRL ≥ 25ng/ml or higher than the normal value of the unit test for HPRL. PRL50ng/ml, about 20% have prolactinoma. PRL100ng/ml, about 50% have prolactinoma, optional pituitary CT or MRI. PRL200ng/ml, microadenomas are often present, pituitary CT or MRI must be done. Decreased PRL: Silhan’s syndrome, use of anti-PRL drugs such as bromocriptine, levodopa, VitB6, etc. (v) T is mildly to moderately elevated in patients with testosterone COS; T is elevated in ovaries or adrenal glands with androgen-secreting tumors and hirsutism.