The most commonly used test to confirm the diagnosis of syphilis in clinical practice is the syphilis spirochete particle agglutination test. This is clinically referred to as TPPA and is the most commonly used one. The same test principle can also be used with the TPHA, called syphilis spirochete hemagglutination time, only the specific way it is taken is different, but the principle is the same. In addition, a fluorescent dense spirochete antibody uptake test can also be used to confirm the diagnosis of syphilis. Other tests, such as the toluidine red unheated serological test and the rapid plasma reactin ring card test, do not use the antigen of the syphilis spirochete, but rather some cardiolipin, lecithin, cholesterol, etc., which cannot be used to confirm the diagnosis of syphilis spirochetes. These tests for syphilis spirochetes are performed using the antigen of the spirochete itself, so a positive result can confirm the diagnosis.