If the test is negative, syphilis cannot be ruled out because the syphilis infection will go through the syphilis window period, which is the period of latent infection. When a suspected syphilis patient is infected with the syphilis spirochete, the syphilis spirochete will reproduce in the patient’s body, but the level of the syphilis spirochete is relatively weak in early patients, and the presence of conventional syphilis serology tests may not be detected, usually within the window period of 40-90 days to do serology tests are often negative, and does not exclude that the patient has been infected with syphilis or whether the infection is syphilis. In clinical practice, doctors recommend that patients be tested 1 month, 3 months, and 6 months after they have had suspected sexual activity or suspected infection, and only if all three tests can rule out syphilis can they be diagnosed as negative for syphilis.