In recent years, assisted reproductive technology has made great strides and many infertility patients have realized their dream of having children. Even in patients with no sperm in the semen, as long as sperm can be found in the testes or epididymis, the egg can be fertilized by intracytoplasmic single sperm microinjection technique. However, there is no good way for patients who have no sperm in their semen and no or very little sperm in their testicles. Since 2009, the Department of Reproductive Medicine at our hospital has been conducting research on germ cell culture. Through years of efforts, it was possible to significantly increase the sperm count in patients with spermatogenic disorders through testicular tissue culture. Using the sperm obtained from culture, it is possible to achieve fertility through IVF technology. The technology was highly evaluated by domestic experts in reproductive medicine, and passed the ethics committee review in September 2012, and was approved to enter clinical application by the hospital new technology committee in October 2012. on March 14, 2013, the Shanxi Provincial Department of Science and Technology organized experts to appraise the results of the germ cell culture technology presided over by Wang Huaixiu, and the participating experts highly evaluated the results and agreed that the results The experts at the meeting highly evaluated the achievement and agreed that the achievement reached the international leading level and the application of the technology will bring gospel to male infertility patients with spermatogenic disorders.