Objective: To analyze the clinical and pathological characteristics of patients with primary biliary cirrhosis (PBC), autoimmune hepatitis (AIH), and PBC/AIH overlap syndrome. Methods: The clinical data of 105 patients with autoimmune liver disease were analyzed to compare the clinical manifestations and liver histopathological changes in patients with PBC/AIH overlap syndrome and those with PBC or AIH alone. Results: Among the 105 patients, including 11 with PBC/AIH overlap syndrome, 60 with PBC, 33 with AIH and 1 with primary sclerosing cholangitis (PSC), there were no significant differences in gender, age, symptoms and complications between patients with PBC/AIH overlap syndrome and those with PBC or AIH (P>0.05), and their laboratory tests had characteristics of AIH, such as serum The laboratory tests were characterized by AIH, such as significantly elevated serum alanine aminotransferase, aspartate aminotransferase, and immunoglobulin IgG; they also had the characteristics of PBC, such as significantly elevated GGT, ALP, and immunoglobulin IgM, but there was no statistical difference compared with PBC or AIH (P>0.05); the autoantibody test showed positive anti-nuclear antibody, anti-mitochondrial M2 antibody, and anti-core protein gP210 antibody. The detection rate of the latter two antibodies was significantly higher than that of AIH patients.