1.Abstinence for 3 to 5 days, not more than 7 days at most for semen analysis. 2. Sperm staining test to distinguish dead sperm from live and immobile sperm. 3. Quantitative examination of white blood cells in semen. 4, Seminal plasma biochemical examination, mainly including prostate acid phosphatase, fructose and α-glucosidase, etc., in order to determine the function of prostate, seminal vesicle glands and epididymis. 5, immunological examination, mainly anti-sperm membrane antibody examination. 6, Reproductive system hidden infection indicator DD seminal plasma elastase examination. 7, Seminal plasma microbiological examination, including mycoplasma and chlamydia examination. 8, endocrine examination, chromosome analysis if necessary. 9, Make sperm function test.