Liver pathology is the gold standard for the diagnosis of liver disease, but it must be performed only if biopsies are obtained, so it is usually performed when the diagnosis needs to be confirmed or the effect of treatment needs to be observed. There are currently three methods of liver biopsy, one is transdermal puncture, which is easy to perform and has fewer risks and complications, but there are sampling errors. Second, direct laparoscopic liver biopsy, the tissue block taken is larger, can be punctured from the obvious lesion, and the bleeding situation can be handled promptly, which is preferred by clinicians, but is more invasive than the former, and patients are less willing to accept it. Third, direct excision of liver tissue in surgical situations is usually performed at the same time as the patient requires certain surgical procedures. Since liver puncture is an invasive test, attention should be paid to its indications and contraindications. Currently, liver puncture biopsies performed in our department are generally percutaneous punctures performed under ultrasound guidance, which are simple and safe to perform. Who needs liver aspiration pathology examination? 1.Patients with chronic hepatitis B and C to determine the treatment plan or to compare the efficacy before and after treatment; 2.Patients with hepatosplenomegaly and unclear diagnosis; 3.Patients with persistent liver function abnormalities of unknown cause; 4.Patients with suspected liver tumor; 5.Patients with difficult liver disease and the diagnosis cannot be confirmed by biochemical and imaging examination; 6.Other diseases that need to be confirmed by liver aspiration biopsy. What kind of people cannot have liver puncture biopsy? 1, abnormal coagulation function, prothrombin time, clotting time is significantly prolonged; 2, the number of platelets is reduced, there is a tendency to bleeding; 3, serious illness with liver failure or severe anemia; 4, uncooperative; 5, other.