The timing of hepatitis B vaccination varies for different groups of people. For newborn infants and young children, the first vaccination of hepatitis B vaccine is required within 24 hours, and the second vaccination of hepatitis B vaccine is required after the first month, and the infants need to be vaccinated with another vaccination of hepatitis B vaccine at the age of six months, and thereafter the titer of hepatitis B surface antibody can be tested at intervals of one month or so to see whether the titer of hepatitis B surface antibody meets the requirement. The vaccination time for adults needs to be judged according to the results of hepatitis B quantitative test, if the test results show that hepatitis B surface antibody is negative or weakly positive, and there is no hepatitis B virus infection, suggesting that the body does not have the ability to resist the virus, and it cannot achieve the need to protect the organism, and at this time, it is possible to take reference to the order of vaccination for young children, and continue to adopt the way of vaccination of hepatitis B booster vaccine in the form of 0, 6, 1, and repeat the examination of the hepatitis B penta1 in about 1-2 months after vaccination to ascertain whether the titer of antibody reaches the corresponding level. Infants and young children should be vaccinated on time after birth in strict accordance with the order of hepatitis B vaccination. Adults are recommended to re-check the quantitative Hepatitis B V test at intervals, and should be vaccinated in time once the Hepatitis B surface antibody titer is found to be lower than normal. In addition, it is recommended to check the quantitative test of hepatitis B V as much as possible, which can determine the protective power of antibody according to the value, and it is more targeted compared with the qualitative weak positive test.