After the initial hepatitis B vaccination, about 30%-40% of the vaccine will produce antibodies after the first dose, about 60%-70% after the second dose, and about 90% after the third dose. After hepatitis B vaccination, the main way to determine whether a booster vaccination is needed is to check the hepatitis B surface antibody. The higher the antibody titer, the stronger the immunity. In the absence of resistance, a three-dose regimen is generally recommended, i.e., 0, 1, and 6 regimens. Usually after the 1st vaccination, by the 2nd dose of hepatitis B vaccine, most people have produced hepatitis B surface antibodies, but in smaller amounts and at lower titers. Therefore, 2 more doses of hepatitis B vaccine are needed to achieve a booster immunization. After the third dose of hepatitis B vaccine, hepatitis B antibody monitoring will be performed at an interval of 1 month, and about 90% of people can develop protective antibodies to effectively prevent hepatitis B virus transmission. The production of hepatitis B antibodies does not mean lifelong immunity, and the five hepatitis B tests need to be repeated every few years. If antibodies are weakened or disappear, the vaccination should be reinforced or revaccinated in a timely manner.