Department of Oncology II successfully implemented the Ar-He knife animal liver freezing experiment on August 18, 2011, which proved the exact and reliable effect of Ar-He knife freezing. Liver before single-needle freezing: Temperature display of -160℃ during argon freezing: Changes of liver after 10 minutes of single-needle freezing: Temperature display of +23℃ after helium rewarming: Changes after dissecting the liver: complete necrosis of liver tissues in the frozen area, about 5*4cm Liver before triple-needle freezing: Changes of liver after triple-needle freezing: Changes of liver after dissecting the liver after triple-needle freezing: fusion of necrotic areas, about 8*4*5cm Before and after freezing Microscopic changes: