Tests for hemolysis include: i. Routine blood tests, such as decreased red blood cells and hemoglobin, and increased peripheral blood nucleated red blood cells, all suggest the possible presence of hemolysis. Serum bilirubin, mainly unconjugated bilirubin is elevated, and jaundice in patients with hemolysis can show dynamic changes in bilirubin levels. Third, fixed blood type, A, B, O hemolytic disease, the mother is O, the newborn is A or B. RH hemolytic disease, the mother is RH negative, the newborn is RH positive, such as the mother is RH positive, the infant RH negative, can also occur anti-E, anti-C, anti-E anti-C caused by the hemolytic disease. Fourth, the anti-human globulin test, both Coomb’s test, checks for specific serum antibodies and can confirm whether the patient’s red blood cells are sensitized by blood group antibodies. If the direct test is positive, it means that the patient’s red blood cells have been sensitized, and the diagnosis can be made after a positive release test. Other auxiliary tests such as urine routine, fecal routine and autoantibodies are also helpful in the diagnosis of hemolysis.