Laparoscopic cholecystectomy is a minimally invasive procedure, starting from the initial 4-hole approach, which has been a revolutionary development compared to traditional open surgery, and to date the majority of laparoscopic cholecystectomies described in classic textbooks in Europe and the United States and in our textbooks have the 4-hole approach. With the proficiency of the technique, nowadays the 3-hole laparoscopic cholecystectomy has become the mainstream approach, reducing the step of an assistant pulling the bottom of the gallbladder and has been able to deal with most of the problems. In recent years, there have been more reports of 2-hole laparoscopic cholecystectomy, which mostly consists of double access from the umbilicus and placement of a gripper to assist in retraction, or fixing the gallbladder to the abdominal wall with a wire, which, compared with the 3-hole method, is difficult for surgical exposure and operation because the direction of retraction cannot be adjusted, inevitably increasing the difficulty and prolonging the operation time, while the former requires special surgical instruments, limiting its application. Therefore, although there are many reports of the 2-hole method, it is difficult to become a mainstream procedure. The basic method is to place two 5-mm puncture cannulas along the subumbilical area, place the laparoscope and electrocoagulation hooks, respectively, and fix the gallbladder base with percutaneous puncture sutures for traction to complete the operation, or to place three channels in the umbilical area and operate with special instruments. This has the advantage of no incisional scar in the upper abdomen, but it is difficult to be promoted because of the technical difficulty of the procedure, the difficulty of the operation, and the need for special instruments. In addition, there are still new methods such as robot-assisted cholecystectomy and cholecystectomy through the natural orifice of the human body, which have not yet been promoted. Throughout the improvement of laparoscopic cholecystectomy incision, it is only to reduce the operating holes and to concentrate the operating instruments in 2 or 1 incision, which inevitably causes the increase of poking holes in other incisions (umbilicus) and the increase of operating difficulties. With the relative maturity of the 3-hole method of laparoscopic cholecystectomy and the already very satisfactory minimally invasive results, it is still worthwhile to discuss in depth how much minimally invasive benefit can be brought by reducing the two 5-mm puncture holes, even if the cosmetic effect is fully considered.