Due to gallbladder stones cholecystitis, acute pancreatitis, and bile duct stone development can all cause discomfort such as pain in the upper middle abdomen. Due to the close anatomical relationship between the biliary tract and the pancreas, the two can also interact with each other when lesions occur. In particular, distal obstruction of the bile duct may cause bile to flow back into the pancreatic duct, activating pancreatic enzymes and leading to the development of acute pancreatitis. This is the pathogenesis of so-called biliary pancreatitis. Therefore, it is necessary to test the serum amylase concentration at the onset of gallbladder stones, bile duct stones, and especially in the diagnosis of acute lesions to prevent missed diagnosis and result in inappropriate selection of therapeutic measures.