With the gradual improvement of the understanding of gallbladder function, endoscopic minimally invasive biliary technology has been widely carried out and popularized, and this technology has been greatly developed in China. The Endoscopic Minimally Invasive Biliary Surgery Committee has enriched and improved the previous Guidelines, so as to provide guidance and help for the implementation of this technology. 1. Gallbladder stones: 1.1. Indications ①Gallbladder stones are diagnosed by ultrasound or other imaging examinations; ②Normal gallbladder function is confirmed by 99TeECT or oral cholecystography; ③The gallbladder is not visualized by 99TeECT, but the stones can be removed intraoperatively and the patency of the gallbladder duct is confirmed. (1) Relative indications ① Acute and subacute stage cholecystitis combined with gallbladder stones: decided according to the intraoperative situation. a: acute congestion and edema of the gallbladder wall tissue, but after decompression of the gallbladder or removal of stones, the gallbladder wall tissue is soft, the tissue level is clear, and there is no ischemic necrosis foci in the gallbladder mucosa, gallbladder drainage can be placed according to the inflammation of the gallbladder, and cholangioscopy can be performed after 4-6 weeks of drainage. If the gallbladder drainage is smooth, the thickness of the gallbladder wall is less than 5 mm, and the gallbladder function is good, the gallbladder stone extraction surgery is feasible. If there is no bile outflow from the fistula, cholangiogram shows gallbladder atrophy, uneven thickness of gallbladder wall and blockage of the gallbladder duct, the gallbladder should be removed; ② gallbladder-filled stones: after gallbladder stones are removed, the gallbladder duct is patent, bile can refill the gallbladder, showing nearly normal gallbladder shape, and the gallbladder wall tissue is soft and the tissue level is clear, bile preservation and stone extraction surgery is feasible. If the gallbladder mucosa is atrophied and the gallbladder wall is fibrotic, cholecystectomy should be performed; ③ white bile, gallbladder atrophy: intraoperative observation of gallbladder emptiness, after removal of stones, the gallbladder duct is clear for bile flow into the gallbladder, and the gallbladder wall tissue is soft and the tissue level is clear, gallbladder stone extraction surgery is feasible; ④ intermural gallbladder stones: endoscopic removal of intermural stones, postoperative inflammatory gallbladder mucosa If the mucosal inflammation of the gallbladder is mild and the contractile function of the gallbladder is normal before surgery, bile-conserving surgery is feasible. For Ⅲ° interstitial stones, if the inflammation of the gallbladder mucosa is heavy or the gallbladder contraction is poor before surgery, in order to prevent flocculent accumulation in the gallbladder and recurrence of stones in the short term, cholecystectomy is feasible; ⑤ Intracavitary separation of the gallbladder: if the diameter of the septum is greater than 5mm, and the mucosa is normal, and the thickness of the gallbladder wall is ≤5mm, biliary preservation surgery is feasible. If the diameter of the septum is less than 5mm and the proximal gallbladder is greater than 5cm, partial resection of the distal gallbladder is feasible, otherwise cholecystectomy should be performed. (2) Contraindications: atrophy of the gallbladder and disappearance of the gallbladder cavity; stones in the gallbladder duct that cannot be detected and removed by endoscopy during surgery; obstruction of the gallbladder duct confirmed by intraoperative imaging and cannot be released; diffuse intermural stones of the gallbladder above Ⅲ°; yellow granuloma of the gallbladder and gallbladder stones with cancer. 2.Gallbladder polyp: 2.1 Indications ①Gallbladder polyp diagnosed by ultrasound or other imaging examinations; ②Polyp diameter >5mm. (1) Contraindications ①Gallbladder polyp carcinoma; ②High-grade intraepithelial neoplasia of gallbladder; ③Hemorrhage of gallbladder after resection and ineffective hemostasis; ④Combined with diffuse adenomyosis of gallbladder; ⑤Gallbladder adenomatous polyp with wide base that cannot be completely cut.