Prostate cancer is the most common malignant tumor of the urological system in men, and its morbidity and mortality are increasing year by year, especially in Shanghai where the population is aging. Ultrasound-guided puncture biopsy is currently the gold standard for the diagnosis of prostate cancer, but with only color ultrasound guidance, it is not only easy to miss some early stage patients, but also easy to underestimate the malignancy of prostate cancer. How to maximize the efficiency of puncture has been the common hope of doctors and patients in various countries. Fortunately, with the continuous advancement of imaging technology, many effective prostate puncture guidance modalities have emerged, among which magnetic resonance diffusion imaging (DWI), which measures the diffusion state of water molecules, and ultrasonography imaging (CEUS), which shows the microvascular situation in the prostate, are particularly effective and have been gradually used in clinical practice. Diffusion-weighted imaging (DWI) DWI is a magnetic resonance functional imaging method that reflects the diffusive motion of water molecules. It can evaluate the diffusion motion of molecules in living tissues non-invasively. Water molecules do unrestricted free diffusive motion in vitro. However, in the human body, cell membranes and macromolecular substances restrict its motion. The DWI imaging principle is essentially the application of a symmetric strong gradient magnetic field in the SE sequence of the MRI scan, and water molecules in different environments show different signal characteristics under the influence of the gradient magnetic field. This principle has been applied to the diagnosis of prostate cancer. The data from foreign scholars showed that the ADC value of prostate cancer area was 1.34±0.38×10-3mm2/s, and the ADC value of non-cancerous tissue was 1.61±0.27×10-3mm2/s . Gibbs et al. made a judgment on the ADC threshold value of prostate cancer, and its sensitivity and specificity reached 84% and 80%, respectively. Shimofusa et al. showed that Conventional T2WI combined with DWI can significantly improve the diagnosis of prostate cancer. The combination of the two diagnostic methods with each other has a high diagnostic value for prostate cancer. According to the literature in 2014, Bains et al. showed that the sensitivity of DWI in the diagnosis of prostate cancer by puncture has reached 89%-91% and the specificity 77%-81%. 72% sensitivity in the study of Le et al. for high-grade prostate cancer can effectively increase the diagnostic efficacy of prostate puncture. Ultrasonography imaging (CEUS) Ultrasonography, also known as acoustic imaging, refers to the injection of contrast agent through a peripheral vein to suspend a large amount of microbubble contrast agent in the blood, which increases the acoustic impedance difference between blood and gas, thus enhancing the backscatter of microbubbles, resulting in enhanced echo signal at the site, improving the signal-to-noise ratio of echo, enhancing the display of tumor microvessels, and improving the detection rate of tumors. The most commonly used gray-scale ultrasound can detect prostate cancer lesions by morphology, but due to the histological characteristics of prostate cancer, the sensitivity and specificity of conventional gray-scale ultrasound for prostate cancer diagnosis are only 35%~55% and 17%~55%. The angiogenesis of the tumor is the basis for the growth, infiltration and metastasis of tumor cells. Compared to normal prostate tissue, prostate cancer lesions have significantly more blood vessels and can show a typical fast-in and fast-out phenomenon. The fast-in phenomenon may be related to the large amount of neovascularization. The tumor blood vessels lack the normal dendritic distribution structure compared with normal blood vessels, and are tortuous and dilated, with uneven thickness and excessive branching, resulting in irregular enhancement on ultrasonography. The fast out phenomenon may be due to arteriovenous short circuit. In the case of benign prostate nodules, there are fewer neovascularization and larger venous vessels, so the discharge of contrast agent is relatively slow, showing a slow exit type. Real-time grayscale ultrasonography has shown its potential in the detection of prostate cancer lesions because of its ability to sensitively show the neovascularization associated with the development of prostate cancer. A number of studies have found that targeted puncture by real-time gray-scale ultrasonography can correspondingly improve the detection rate of prostate cancer and avoid unnecessary repeat punctures. Although the current results of both techniques in guiding prostate puncture biopsy are promising, both have certain shortcomings. Experimental data suggest that there is a large crossover area in ADC values between prostate cancer and benign lesion tissues, which increases the difficulty of early diagnosis of focal carcinoma, thus generating a partial false positive rate and reducing the sensitivity and specificity of the diagnosis. DWI is also prone to false-positive results in the presence of high cell density in areas of benign prostatic hyperplasia. Similarly, in the study by Le et al, it was found that the sensitivity of DWI was only 47% for low-grade prostate cancer. Also the ADC values for determining cancer foci vary widely among the results of the studies. Currently, clinical data are limited and there is no uniform standard for diagnosing prostate cancer with ADC values for the time being. In contrast, CEUS can only provide dynamic temporal observation of a specific section, which can easily lead to missed diagnosis when the prostate volume is too large or the cancer is in a special location, etc. Currently, the enhancement time (AT), time-to-peak (TTP) and peak intensity (PI) in the ultrasonography of CEUS are very important for prostate cancer at home and abroad. intensity (PI) are also controversial for the diagnostic criteria of benign and malignant prostate cancer. We propose to combine these two techniques to develop better individualized prostate puncture protocols for patients, hoping to effectively compensate for the shortcomings of both, reduce the number of puncture needles without increasing the rate of missed diagnoses, reduce patient pain, and increase the efficiency of puncture. The use of both in the development of individualized prostate puncture protocols has not been reported in the domestic and international literature. It is hoped that this study will not only benefit patients, but also provide a preliminary standard for identifying benign and malignant prostate lesions with ADC, AT, TTA, PI and other data.