Semen contains a variety of substances, such as high concentrations of organic substances, inorganic ions and various enzymes. Among them, many enzymes related to semen coagulation or liquefaction come from prostatic fluid, such as aminopeptidase, fibrinolytic enzyme, arginine ester hydrolase, etc. In addition, citric acid is all secreted from the prostate gland, and its role is to maintain the osmotic pressure of semen and the activity of sperm hyaluronidase. The common causes of semen non-liquefaction are insufficient fibrinolytic enzymes secreted by the prostate due to seminal vesiculitis and prostatitis; deficiency of trace elements (magnesium, zinc, etc.); congenital prostate deficiency, etc. The secretions of the prostate and seminal vesicles are involved in the process of coagulation and liquefaction of semen. The coagulation factors produced by the seminal vesicles cause the semen to coagulate, while the liquefaction factors produced by the prostate, such as proteolytic enzymes and fibrinolytic enzymes, cause the semen to liquefy. Once inflammation occurs in the seminal vesicles or prostate, the secretion of the above factors may be impaired, resulting in an increase in coagulation factors or a decrease in liquefaction factors, resulting in semen non-liquefaction. The prostate gland and seminal vesicles are both open to the seminal mound, and about 60% of patients with prostatitis are complicated by seminal vesicles, leading to hematospermia and painful ejaculation; the prostate fluid secretion decreases during prostatitis, so the semen volume also decreases, and the low semen volume is not conducive to the survival and activity of sperm. The activity of enzymes in the prostate fluid decreases and the coagulation factor increases, so the liquefaction time is prolonged after the semen is discharged. The acidity (pH) value of semen decreases, from alkaline to acidic. When the pH value drops to 6-6.5, the sperm viability decreases, resulting in dead sperm or weak sperm. More harmful substances such as bacteria and bacterial toxins and inflammatory secretions in prostate fluid can consume nutrients and oxygen in semen, thus affecting sperm survival and motility and leading to infertility.